Fig. 6.

P2Y receptor agonist 2-methylthio-ADP (2-meSADP) does not evoke any current in adult WT mouse cardiac myocytes. A: 2-meSATP, but not 2-meSADP, was able to induce a steady inward current in an adult (3-mo-old) mouse cardiac myocyte held at − 80 mV. Both agonists were present at 3 μM. The current induced by 2-meSATP reversed upon washout of the agonist. The vertical marks on the current trace are ramp voltage clamps from −100 to +50 mV. B: The I-V relations taken at a and b (for 2-meSADP) and at a and c (for 2-meSATP) in A were subtracted, and the differences were plotted as a function of the voltage in pA/pF. Data were shown as means ± SE from 8 WT cardiac myocytes from 14 mice.