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. Author manuscript; available in PMC: 2018 Nov 25.
Published in final edited form as: Mucosal Immunol. 2017 Aug 9;11(2):549–561. doi: 10.1038/mi.2017.70

Figure 5. dmLT promotes a balanced Th1/Th17 transcriptional phenotype in antigen-specific CD4+ T cells following intradermal immunization.

Figure 5.

Mice were intradermally immunized with CpG or dmLT plus 2W1S-GFP and the 2W1S-specific CD4+ T cell transcription factor expression was assessed on day nine. CLN and spleen were harvested, dissociated, and immediately stained for the intracellular transcription factors T-bet and Rorγt. A) representative histograms of each transcription factor are shown including the isotype control. B) Graphs represent the percent of 2W1S-specific T cells expressing each transcription factor in immunized groups using 5 mice per group. Results are representative of four independent experiments. Significance was determined by student’s two-tailed t-test. Statistical significance is defined as follows: *, P<0.05; **, P<0.01; ***, P<0.005. Error bars represent SEM.