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. 2018 Nov 19;9:2775. doi: 10.3389/fmicb.2018.02775

Table 1.

Sequencing statistics and various alpha-diversity metrics for the subset of 16S rRNA gene fragments assigned to the Acidobacteria.

Sample No Raw reads Filtered reads Reads from Acidobacteria Diversity indices
Chao1 Shannon Observed species∗∗ Menhinick Good’s coverage estimator
1 128,572 112,642 29264 837.8 5.4 550 3.2 0.992
2 402,892 356,887 91919 (34000) 1070.8 (1014.7) 5.6 (5.6) 974 (685) 3.2 (3.7) 0.997 (0.992)
3 151,288 136,837 36224 855.2 5.3 612 3.2 0.993

Filtered reads: number of sequences excluding singletons and chimera sequences. ∗∗Calculations are based on a sequence similarity cut-off of 97%. The randomized number of sequences is given in parentheses for the overrepresented sample 2.