Table 1.
Sequencing statistics and various alpha-diversity metrics for the subset of 16S rRNA gene fragments assigned to the Acidobacteria.
Sample No | Raw reads | Filtered reads∗ | Reads from Acidobacteria | Diversity indices |
||||
---|---|---|---|---|---|---|---|---|
Chao1 | Shannon | Observed species∗∗ | Menhinick | Good’s coverage estimator | ||||
1 | 128,572 | 112,642 | 29264 | 837.8 | 5.4 | 550 | 3.2 | 0.992 |
2 | 402,892 | 356,887 | 91919 (34000) | 1070.8 (1014.7) | 5.6 (5.6) | 974 (685) | 3.2 (3.7) | 0.997 (0.992) |
3 | 151,288 | 136,837 | 36224 | 855.2 | 5.3 | 612 | 3.2 | 0.993 |
∗Filtered reads: number of sequences excluding singletons and chimera sequences. ∗∗Calculations are based on a sequence similarity cut-off of 97%. The randomized number of sequences is given in parentheses for the overrepresented sample 2.