Table 3.
Disease severity (DS) LSMean values obtained from CLMM analysis of DS ratings on roots of eight genotypes of four different legume species, in response to inoculation with 34 A. euteiches isolates from the collection (two isolates per field).
| Species | Pea | Vetch | Faba bean | Alfafa | Virulence phenotype | Pathotype | MLG | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| Isolates | LSMeans Lumina | LSMeans MN313 | LSMeans PI180693 | LSMeans Amethyste | LSMeans Topaze | LSMeans Baraca | LSMeans Melodie | LSMeans Zenith | |||
| RB84 | 12.328 | 6.813 | 2.236 | 4.119 | −3.785 | 1.111 | −2.143 | 1.274 | PVFA | I | 3 |
| RB3 | 5.892 | 4.830 | −0.537*** | −0.391*** | −1.729 | −1.861*** | −5.595 | −1.150*** | PVFA | I | 3 |
| RB5 | 8.316 | 5.616 | 1.089 | 0.542*** | −3.856 | −1.899*** | −5.615 | −0.071 | PVFA | I | 1 |
| BI1 | 7.285 | 6.764 | 0.614 | 1.612 | −4.255 | −2.261*** | −3.853 | 0.067 | PVFA | I | 4 |
| BI6 | 8.316 | 7.845 | 1.292 | 1.565 | −4.652 | −1.655 | −3.294 | 0.168 | PVFA | I | 1 |
| BRO-2 | −1.013 | −3.535*** | −4.583*** | −9.432*** | −2.320 | −5.620*** | −5.623 | −5.468*** | P | I | 1 |
| BRO-6 | 0.444 | −1.891*** | −2.775*** | −7.566*** | −1.547*** | −5.535*** | −5.603 | −4.034*** | P | I | 1 |
| BR1-2 | 2.452 | 3.888 | −0.753*** | 1.121 | −3.426 | 0.494 | −2.671 | 2.258 | PVFA | I | 12 |
| BR1-3 | 5.396 | 5.975 | −0.762*** | −0.654*** | −2.977 | 0.649 | −1.644 | 1.650 | PVFA | I | 11 |
| BR2-1 | 7.105 | 5.752 | 0.994 | −3.603*** | −5.411 | −1.829*** | −3.510 | −0.027 | PVFA | I | 1 |
| BR2-4 | 9.035 | 6.675 | 0.923 | −6.639*** | −4.555 | −2.508*** | −5.656 | −2.495*** | PFA | I | 1 |
| BR3-1 | 5.547 | 5.901 | −1.939*** | 3.124 | −4.271 | 1.175 | −1.665 | 1.804 | PVFA | I | 11 |
| BR3-5 | 5.749 | 5.978 | −1.003*** | −0.949*** | −3.497 | 0.707 | −0.765 | 0.009 | PVFA | I | 10 |
| HBO-1 | 8.175 | 4.128 | −0.130 | −1.640*** | −4.317 | −1.401 | −4.028 | 1.939 | PVFA | I | 1 |
| HBO-9 | 9.012 | 4.981 | 1.829 | 1.991 | −3.003 | −0.018 | −2.898 | −0.617 | PVFA | I | 1 |
| HB1-3 | 7.536 | 6.546 | −0.954*** | −1.058*** | −4.687 | −1.396 | −3.355 | −1.135 | PVFA | I | 1 |
| HB1-14 | 8.982 | 5.966 | 4.200 | 0.919 | −5.411 | −0.305 | −1.313 | 0.560 | PVFA | I | 1 |
| BL5 | 8.941 | 5.617 | 2.057 | 3.666 | −3.294 | 1.002 | −3.260 | 0.485 | PVFA | I | 1 |
| BL8 | 9.012 | 4.371 | 1.713 | 1.414 | −3.679 | −0.575 | −3.826 | 0.969 | PVFA | I | 1 |
| P8 | 8.292 | 5.737 | 0.242 | 0.969 | −4.688 | −0.978 | −2.772 | 0.181 | PVFA | I | 1 |
| P14 | 8.966 | 4.687 | 0.610 | 0.919 | −4.688 | −0.365 | −3.547 | −0.357 | PVFA | I | 7 |
| F3 | 9.035 | 6.081 | 3.466 | 2.439 | −5.447 | 0.377 | −1.589 | −0.264 | PVFA | I | 8 |
| F10 | 8.959 | 7.125 | 2.641 | 2.133 | −4.634 | 0.339 | −1.074 | −0.123 | PVFA | I | 8 |
| ENO-12 | 8.975 | 7.479 | 1.317 | 2.298 | −3.954 | 0.634 | −0.850 | 1.663 | PVFA | I | 4 |
| ENO-16 | 8.064 | 5.592 | 1.121 | 2.466 | −3.570 | 0.211 | −2.630 | 2.494 | PVFA | I | 5 |
| EN1-3 | 8.175 | 7.312 | 2.270 | −8.745*** | −3.109 | −0.861 | −3.242 | 1.825 | PFA | I | 7 |
| EN1-5 | 9.035 | 6.196 | 0.451 | 1.633 | −3.131 | 0.495 | −2.399 | 0.582 | PVFA | I | 1 |
| NR8 | 9.012 | 4.660 | 1.087 | 1.979 | −4.707 | −1.776*** | −2.198 | −0.240 | PVFA | I | 1 |
| NR14 | 8.982 | 6.262 | 2.903 | 2.411 | −3.952 | 0.605 | −2.097 | 0.095 | PVFA | I | 1 |
| MO1 | 7.853 | 6.783 | 1.920 | 0.843 | −4.254 | −1.298 | −4.846 | 0.510 | PVFA | I | 1 |
| MO5 | 9.006 | 5.584 | 2.487 | 0.558*** | −5.395 | −1.065 | −4.914 | 0.003 | PVFA | I | 6 |
| BO5 | 7.291 | 5.211 | 1.982 | −11.334*** | −4.271 | −2.422*** | −2.769 | −0.673 | PFA | I | 7 |
| BO13 | 7.105 | 5.932 | 2.507 | −10.555*** | −3.698 | −1.906*** | −3.890 | −1.883*** | PFA | I | 7 |
| CR5 | 9.035 | 4.925 | 0.390 | −1.097*** | −5.410 | −0.168 | −2.871 | 0.770 | PVFA | I | 1 |
| CR9 | 8.989 | 6.161 | 1.636 | 1.427 | −4.688 | 0.252 | −2.435 | 0.515 | PVFA | I | 1 |
| Mean | 7.580 | 5.370 | 0.873 | −0.672 | −4.008 | −0.847 | −3.155 | 0.037 | |||
| Standard Deviation | 2.556 | 2.234 | 1.780 | 4.211 | 0.986 | 1.622 | 1.432 | 1.640 | |||
LSMean scores highlighted in gray with the isolates tested are significantly different from the LSMean score obtained with the RB84 reference strain for the same genotype (***p-value < 0.001). A Virulence phenotype is defined on pea (P), vetch (V), faba bean (F) and alfalfa (A) when the probability of DS score ≥ 1 on the four susceptible legumes (Pea: Lumina; Vetch: Amethyste; Faba bean: Baraca; Alfalfa: Zenith) is equal or higher than 0.5, according to the CLMM analysis. Pathotype I was attributed for all the isolates since LSMeans scores between the three pea genotypes were significantly different (p-value < 0.05), with Lumina, MN313 and PI180693 being the susceptible, intermediate and partially resistant genotypes, respectively (Additional File 8). MLGs (Multilocus groups) are indicated according to the genetic diversity analysis conducted in this study.