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. 2018 Nov 19;9:1633. doi: 10.3389/fpls.2018.01633

FIGURE 1.

FIGURE 1

CaWRKY27 was transcriptionally induced by heat treatment in pepper plants. (A) CaWRKY27 expression in pepper leaves was determined with qRT-PCR at the indicated time points during or after heat treatment. Relative transcript levels of CaWRKY27 in heat-treated pepper plants were compared to the control, which was set to a relative expression level of ‘1.’ Data represent the mean ± SE of three biological replicates, and asterisks indicate significant difference compared to control plants (SNK-test, P < 0.05 or ∗∗P < 0.01). H, heat; R, recovery. (B) GUS expression in transgenic Arabidopsis plants carrying the pCaWRKY27::GUS construct. Figures a, b, c1, and c2, show a seedling of 3 (a), 5 (b) and 7 (c1 and c2) days after germination (DAG). In figures d1 and d2 transgenic pCaWRKY27::GUS seedlings were heat treated. In figures d1-shoot, d1-root, d2-shoot, and d2-root the shoot or root was magnified from the corresponding d1 or d2 figures, respectively. These seedlings or plants were grown on ½ MS media under 16 h light/8 h dark conditions.