FIGURE 3.

Overexpression of CaWRKY27 reduced basal thermotolerance in transgenic Arabidopsis plants. (A) Leaves of CaWRKY27-overexpressing Arabidopsis and WT plants that were grown in pots for 5 weeks under normal conditions. (B) Phenotypes of CaWRKY27-OE10, OE12, and WT seedling that were treated at 37°C for 4 h and then returned to 22°C for 2 days of recovery. (C) Phenotypes of 35-day-old CaWRKY27-OE10, OE12, and WT plants that were treated at 37°C for 6 h and then returned to 22°C for 3 days of recovery. (D) Survival rate were analyzed in CaWRKY27-OE10, OE12, and WT plant after 3 days recovery following heat treatment at 37°C for 6 h. Data represent the mean ± SE (n = 3), each replicate consists of eight plants. (E) Electrolyte leakage in 28-day-old heat-treated seedlings was monitored by measuring conductivity. (F) H₂O₂ accumulation in leaves from heat-treated (37°C) WT, CaWRKY27-OE10, and OE12 plants was measured by DAB staining. Data represent the mean ± SE of three biological replicates. Different letters indicate significant differences compared to WT (SNK-test, lowercase letters indicate P < 0.05, and uppercase letters indicate P < 0.01).