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. 2018 Nov 19;9:1633. doi: 10.3389/fpls.2018.01633

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Copyright © 2018 Dang, Lin, Xue, Chen, Guan, Wang and He.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Analysis of defense-associated gene expression in CaWRKY27-silenced plants. The silenced (PYL-279-wrky27 and PYL-279-wrky27-3’utr) and control (PYL-279) pepper plant leaves were harvested after 0 and 2 h at 37°C, and after 6 h of recovery at 25°C (after 2 h of heat treatment). (A–E) Relative transcript levels of genes encoding the ROS-scavenging enzymes CaCAT1, (A); CaAPX1, (B); CaAPX2, (C); CaCSD2, (D); and CaSOD1, (E). The transcript level of each gene was determined by qRT-PCR and normalized against CaActin, followed by normalization against the transcript level of the gene in PYL-279. Data represent the mean ± SE of three biological replicates. Different letters indicate significant differences compared to the control (SNK-test, lowercase letters indicate P < 0.05 and uppercase letters indicate P < 0.01).