(a) Males had a greater change in mass after leg loss compared to females; mass lost corresponded to mass of leg lost (GLM: Wald χ² = 29.27, d.f. = 1,54, p = 0.029, n males = 43, n females = 15). (b) Female metabolic rate dropped after leg loss, but not significantly, while male metabolic rate dropped significantly after leg loss (GLM: Wald χ² = 6.07, d.f. = 1,54, p = 0.017). Each point on the graph represents mean values for females (grey) and males (orange); dashed lines and error bars show standard errors. (Online version in colour.)