Table 5.
Parameters of ELISA for AP.
| Immunogen | BSA-C6-alkylphenol |
| Tracer | HRP-C6-alkylphenol |
| Detection limit | 1 nM |
| Working range | 2 – 100 µM |
| Precision | |
| Intra-assay | 1 – 50 % C.V. |
| Recovery | 20 – 95 % * |
| Cross-reactivity (%) | |
| Octylfenol | 100% |
| 4-tert-Butylphenol | 6% |
| 2-sec-Butylphenol | 1% |
| 4-pentylphenol | 556% |
| 4-n-heptylphenol | 256% |
| 4-n-propylphenol | 33% |
| 2-n-propylphenol | <0.1% |
| 4-Isopropylphenol | 6% |
| 4-n-hexylphenol | 300% |
| 4-chloro-2-cyclo-hexylphenol | < 0.1% |
| Nonylphenol (tech) | 12% |
| 4-n-nonylphenol | 30% |
| Bisphenol A | 8% |
* = depending on the matrix.