Table 2.

Sequences of the primers.

PCR primer	5′ → 3′	PCR region	Anneal temp	Product length
Exon-1F DSG3	GGCCTCTTTTGCTTGTGAGAAA	25786301	61	1571
Exon-1R DSG3	CAGTCACGATCAAACGTTGAGTT	25784966
Exon-2F DSG3	CAGTAGCCGGATTTTGGCAG	25788145	60	528
Exon-2R DSG3	ACTTGGCATGTGCTCTTGGA	25787897
Exon-3F DSG3	ACCGATTCATTGAAACCCTGTT	25791050	61	412
Exon-3R DSG3	CTCCTGACCATCAAGGACCA	25790985
Exon-4F DSG3	GGTCCTTGATGGTCAGGAGTC	25791491	64	331
Exon-4R DSG3	TCCAACAATCGCCCTGTCAA	25791351
Exon-5F DSG3	AACATACACGACCTGCTCTGC	25794845	63	600
Exon-5R DSG3	AACCCCAACAGCCCTCATAA	25794593
Exon-6F DSG3	TGCTCCCAAATGGACTCCC	25796339	63	365
Exon-6R DSG3	GCACAACTTGCTCTGGATAGTTC	25796111
Exon-7F DSG3	GCAGTGATAAAGGTAATGTGTTAT	25798279	60	422
Exon-7R DSG3	GCAGGAAATTTAAAACCCAGGCT	25798137
Exon-8F DSG3	ATGAGGCAACTTCGCCAGTG	25799330	60	337
Exon-8R DSG3	GAGTTCTCTGGTCCTCTTACCTG	25799057
Exon-9F DSG3	TGTGCTTAAAACAGGCACACT	25802312	59	599
Exon-9R DSG3	AGGGACCAGCAAAGAGACAAT	25802125
Exon-10FDSG3	GCAAGCCAGCAAATCCTGAA	25802738	60	415
Exon-10RDSG3	CCCCACAGGCTTTGTCTACG	25802604
Exon-11FDSG3	ACCCCTCTTCCTTCCCTCTTT	25803899	59	411
Exon-11RDSG3	GTGACACGTAGATTTTTGGCAC	25803733
Exon-12FDSG3	TGGCAGTGGGTAAGTAGCTC	25804674	60	514
Exon-12RDSG3	GAAGGGGCAACTTGGACAAC	25804524
Exon-13F DSG3	ATTCACGCACTGATATCCTTCT	25805338	59	324
Exon-13R DSG3	GACTTGAGAGTGTGGAGCAC	25805217
Exon-14FDSG3	TGTTCTATGGACCCCTCAATCT	25807166	59	400
Exon-14RDSG3	TGCATGATAGTGTCAGGGAGAG	25807028
Exon-15FDSG3	CACAAATACCGGTTTATCTGTTGA	25807700	51	366
Exon-15RDSG3	TGAACAGCAGAATGTAAAAATCAA	25807664
Exon-16F DSG3	ATCTGGCTTGGTTTTCCATGTG	25817374	60	472
Exon-16R DSG3	GAGCAAAAGTGATGCAAGCGT	25817194