Table 6.

Predications of non-synonymous SNPs function by SIFT software.

nsSNPs	Positions	Reference allele	Mutant allele	Amino acid changed	Predications	Score
SNP1	597	C	T	R597E	Affect protein function	0.000
SNP2	591	G	C	T595I	Affect protein function	0.000
SNP3	572	T	C	G572S	Affect protein function	0.000

Amino acid substitutions changed in Tibetan goat protein sequences may have been predicted to modify the function of DSG3 gene (low certainty). The cutoff value in the SIFT program is a tolerance score of ≥ 0.05. The higher the tolerance score, the less functional impact a particular amino acid substitution is expected to have.