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. 2018 Nov 26;25:57. doi: 10.1051/parasite/2018055

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© H. Wang et al., published by EDP Sciences, 2018

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure S3. — rEgTPx expression and Dot-blot analysis of the purified mouse anti-rEgTPx serum. A, Agarose gel electrophoresis image of the PCR-amplified EgTPx gene. Lane1: DNA Marker/DL2 000 (Takara, Otsu, Japan); Lane2: EgTPx was successfully amplified; Lane3: Without template as native control. B, SDS-PAGE analysis of rEgTPx. M: Protein markers; Lane 1: Purified rEgTPx protein. C, Dot-blot analysis of the EgTPx antiserum before purification. D, Dot-blot analysis of the EgTPx antiserum after purification; lanes 1, 2 and 3 show the one-third serial dilutions of purified rEgTPx (upper) and the lysates of bacterial cells transformed with the pET-28a expression vector expressing the His tag (lower).