Figure 4.

Angiomotins function as scaffolds for the Hippo pathway in vivo. A, HEK293 (WT) and Amot-3KO HEK293 cells were grown to high density and serum-starved for 4 h. Immunoprecipitation (IP) was performed on cell lysates with either anti-LATS1 or control (IgG) antibodies, and immune complexes and cell lysates were analyzed by Western blotting for AMOT, SAV1, LATS1, and YAP levels. LATS1 bg, residual LATS1 signal after stripping. B, HEK293 (WT) and Amot-3KO HEK293 cells were grown as in A. Immunoprecipitations were performed on cell lysates with either anti-SAV1 or control (IgG) antibodies, and immune complexes and cell lysates were analyzed by Western blotting for AMOT, MST1, SAV1, LATS1, and YAP levels.