Figure 6.

Time-course of PI-PLC–mediated hydrolysis of [³H]PI in nhTMEM16 and TE-containing proteoliposomes. A, liposomes (filled circles) and nhTMEM16 proteoliposomes (open squares) at PPR of ∼1 mg/mmol containing trace amounts of [³H]PI were prepared as Fig. 5. The extent of PI-PLC–mediated hydrolysis of [³H]PI was determined at each time point as described in Fig. 2A. The data are from two independent experiments. B, liposomes (filled circles) and TE-containing proteoliposomes (open squares) at PPR of ∼10 mg/mmol containing trace amounts of [³H]PI were prepared as Fig. 3. The extent of PI-PLC–mediated hydrolysis of [³H]PI was determined at each time point as described in Fig. 2A. The data are from two independent experiments.