Figure 4.
Modification of cortex structure and spore morphology does not alter chemical resistance. Spore measurements (A) were obtained for pure spore suspensions of the parental (blue) and pdaA1 mutant strain (green). Solid bars indicate the measured length (left axis, nm), and the dotted bars indicate the calculated volumes (right axis, nm3). Chemical and heat sensitivities of purified spore suspensions (B) were obtained for three independent biological replicates of pure spore suspensions: 630Δerm(pMTL84151) spores in blue, ΔpdaA1(pMTL84151) spores in green, and the complemented mutant spores ΔpdaA1(pCH67) strain in gray. *, Student's t test, p < 0.05.