Table 2.

Restriction assay of Trim5 and TrimCyp mutants in the presence and absence of proteasome inhibitor MG132

The cells were transduced with vectors expressing YFP and either TRIM5, TRIMCyp, or their mutants before challenging with HIV-1 expressing GFP, in the presence or absence of MG132. The cells were analyzed by flow cytometry 48 h after challenge. The numbers are ratios of percentages of infected cells containing restriction factor to percentages of infected cells that do not contain restriction factor.

	Without MG132	1 μg/ml MG132	16 μg/ml MG132
Trim 5α	0.12 ± 0.06	0.14 ± 0.04	0.12 ± 0.01
Trim 5α W196A/E197A	0.10 ± 0.05	0.11 ± 0.05	0.09 ± 0.03
Trim 5α E197A	0.12 ± 0.03	0.12 ± 0.04	0.19 ± 0.03
TrimCypA	0.10 ± 0.06	0.10 ± 0.02	0.09 ± 0.02
TrimCypA W196A	0.08 ± 0.03	0.08 ± 0.05	0.05 ± 0.03
TrimCypA W196A/E197A	0.10 ± 0.06	0.11 ± 0.07	0.05 ± 0.01
TrimCypA E197A	0.12 ± 0.05	0.13 ± 0.08	0.11 ± 0.03