Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Oct 1;293(47):18123–18137. doi: 10.1074/jbc.RA118.004530

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Richards et al.

Author's Choice—Final version open access under the terms of the Creative Commons CC-BY license.

PMC Copyright notice

Figure 2. — S-layer proteins of C. difficile Ox247 and mutants. Strains were cultured in BHIS broth, and the S-layer was prepared using low pH glycine extraction. The LMW (▷) and HMW (▶) S-layer proteins of strain 630 are indicated. The 20-kDa LMW SLP (→) predicted in Ox247 is not found in the WT strain, but is found in some but not all mutants. The ∼45-kDa bands in Ox247 analyzed by MS are indicated (**). M, M_r markers; lane 1, 630; lane 2, Ox247 WT; lane 3, orf2::erm; lane 4, orf2::erm (pAAM008); lane 5, orf3::erm; lane 6, orf2::erm (pEJR004); lane 7, orf4::erm; lane 8, orf4::erm (pEJR007); lane 9, orf7::erm; lane 10, orf16::erm lane 11, orf16::erm (pEFR009); lane 12, orf19::erm; and lane 13, orf19::erm (pAAM011).