Skip to main content
. 2018 Nov 19;9:2268. doi: 10.3389/fimmu.2018.02268

Figure 2.

Figure 2

TAG72-BBζ CAR T cells show antigen-dependent cytokine production and tumor killing in vitro. (A) Flow cytometric analysis of TAG72 surface expression on multiple ovarian and colorectal (LS174T) cancer cell lines. (B) Quantification of tumor killing by TAG72-BBζ CAR T cells relative to Mock at an E:T ratio of 1:1, following a 24 and 72 h co-culture with antigen-positive and -negative tumor targets as described in Materials and Methods. (C) TAG72-BBζ CAR T cell expansion at 24 and 72 h following co-culture with indicated tumor targets at an E:T ratio of 1:1. (D,E) IFNγ and IL-2 levels in supernatant quantified by ELISA from Mock or TAG72-BBζ CAR T cells following a 24 and 72 h co-culture with indicated tumor targets at an E:T ratio of 1:1. (F) Flow cytometric analysis of TAG72 surface expression on primary human ovarian cancer cells harvested from patient ascites (OAS) after 72 h in culture. (G) Quantification of tumor killing and (H) IFNγ production by TAG72-BBζ CAR T cells relative to Mock following a 72 h co-culture with freshly thawed OAS cells at an E:T ratio of 1:1.