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. 2018 Nov 19;9:2268. doi: 10.3389/fimmu.2018.02268

Figure 3.

Figure 3

Regional intraperitoneal delivery of TAG72-BBζ CAR T cells significantly reduces tumor burden and extends overall survival of OVCAR3 tumor-bearing mice. (A) Schematic illustrating i.p. engraftment of 5.0 × 106 OVCAR3(eGFP/ffluc) tumor cells in NSG mice, followed by either i.v. or i.p. delivery of 5.0 × 106 Mock or TAG72-BBζ CAR T cells on day 14 post-tumor injection. (B) Representative bioluminescent flux imaging of mice treated i.v. or i.p. with Mock or TAG72-BBζ CAR T cells. (C) Quantification of flux (each mouse) from OVCAR3(eGFP/ffluc) tumor-bearing mice treated i.v. or i.p. with Mock or TAG72-BBζ CAR T cells. N = 3–4 per group. Data are representative of two independent experiments. (D) Kaplan–Meier survival for Mock and TAG72-BBζ CAR T cell treated mice. N ≥ 4 mice per group. Combined data from two independent experiments. (E) Quantification of TAG72-BBζ CAR T cells per μL blood at 6, 13, and 29 days post-treatment. N = 4 per group. (F) Representative flow cytometric analysis of the frequency of human CD45+ (hCD45) and mouse CD45+ (mCD45) cells in the i.p. cavity of tumor-bearing mice at day 6 and 13 post-treatment. Representative images from two independent experiments. ***p < 0.001.