TABLE 1:
Overview of the strains used and the observed defects in cilia entry, IFT, and axonemal binding of IC2-NG.
Strain | Protein encoded by wild-type gene | Complex/process | Amount in flagella | Transport by IFT | Axonemal binding | Presence at base |
---|---|---|---|---|---|---|
oda6 IC2-NG (control) | +++ | +++ | +++ | + | ||
oda2 oda6 IC2-NG | HCγ | ODA subunit | (+) | (+) | – | - |
oda3 oda6 IC2-NG | DC1 | docking complex | (+) | +++ | –* | + |
oda8 oda6 IC2-NG | ODA8 | ODA maturation | (+) | + | (+) | (+) |
oda10 oda6 IC2-NG | ODA10 | ODA maturation | + | (+) | + | n.d. |
oda16 oda6 IC2-NG | ODA16 | ODA transport | ++ | (+) | +++ | (+) |
ift46 IFT46ΔN oda6 IC2-NG | IFT46 | ODA transport | + | (+) | +++ | +++ |
oda16 ift46 IFT46ΔN oda6 IC2-NG | ODA16 IFT46 | ODA transport | + | (+) | +++ | – |
+++, frequent; +, infrequent; (+), rarely observed; –, not observed; *transient docking was observed; n.d., not determined.