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FAK activity and regulation of tractions. (A) Biochemical inhibition of FAK activity and (B) resulting phase-dependent reduction of traction. (C) Average traction in phosphomimetic (Y2E) and nonphosphorylatable (Y2F) paxillin mutants. n = number of cells and n’ = number of independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.
