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. 2018 Oct 15;29(21):2578–2590. doi: 10.1091/mbc.E18-04-0258

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© 2018 de Bruyn Kops et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 1: — The brr6-1 mutant and the NLS-Brr6N fragment impair PAB1 expression. (A) Fluorescence microscopy localizing Pab1GFP in live isogenic WT (wild-type, yDBK398) and b6 (brr6-1, yDBK399) cells. Arrowheads indicate cells with little Pab1GFP protein signal. Plots show quantitation of Pab1GFP levels by flow cytometry. (B) Pab1GFP in wild-type cells (W303) carrying empty vector (pJL602), B6N (pP_{GAL_}NLS-BRR6N-FLAG), or ∆C4N (pP_{GAL_}NLS-brr6∆C4N-FLAG) grown in raffinose media then induced with galactose O/N.