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. 2018 Oct 15;29(21):2578–2590. doi: 10.1091/mbc.E18-04-0258

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© 2018 de Bruyn Kops et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 3: — brr6-1 and the NLS-Brr6N fragment impair PAB1 and GAL1-10 locus positioning. (A, B) Locus positioning assay showing fraction of PAB1 locus at the nuclear rim in homozygous diploid LAC O:PAB1 cells (A) WT (wild type, yDBK523) vs. b6 (brr6-1, yDBK524) and (B) wild-type (W303) cells carrying B6N (pP_{GAL_}NLS-BRR6N) or ∆C4 (pP_{GAL_}NLS-brr6∆C4N) fragment constructs. (C) Growth of WT (wild-type, yDBK165) and b6 (brr6-1, yDBK166) strains on YEP media containing glucose or galactose. (D) Locus positioning assay for GAL1-10 locus in homozygous diploid LAC O:GAL1 cells (WT [wild type, yDBK535] vs. b6 [brr6-1, yDBK536]). Asterisks indicate p value ≤6 × 10^–5.