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. 2018 Oct 15;29(21):2522–2527. doi: 10.1091/mbc.E18-06-0362

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© 2018 Higuchi-Sanabria et al. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.

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FIGURE 3: — HSF-1 is required for maintenance of cytoskeletal integrity. Cytoskeletal integrity was monitored in muscles (A), intestine (B), and hypodermis (C) of transgenic worms expressing LifeAct::mRuby under tissue-specific promoters. Transgenic worms were grown on either EV or hsf-1 RNAi from hatch. Worms were aged by three different methods: flotation, FUDR treatment, or hand picking. Representative data from hand picking are shown. Imaging was stopped at D7 due to a large number of deaths in worms grown on hsf-1 RNAi beyond D10. (D) Cytoskeletal integrity was measured in muscles of wild-type and myo-3p::hsf-1 worms at D1, D4, D7, D10, and D13. (E) Cytoskeletal integrity was measured in the hypodermis of wild-type and col-19p::hsf-1 worms at D1, D4, D7, D10, and D13. (F) Cytoskeletal integrity was measured in the intestines of wild-type and gly-19p::hsf-1 worms at D1, D4, D7, D10, and D13. For A–C, worms were plated on EV RNAi from hatch and aged by flotation, growth on FUDR, or hand picking. Representative images from hand picking are shown. Scale bars are 5 µm.