Fig. 2.

Differentially regulated expression of the mucin gene by 17β-estradiol (E2) supplementation in azoxymethane/dextran sulfate sodium (AOM/DSS)-induced colitis in mice. (A) AOM/DSS-mediated reductions in mucin 2 (MUC2) mRNA expression were enhanced by E2. (B) AOM/DSS-induced expression of MUC4 mRNA was inhibited by E2. Expression of MUC2 and MUC4 in the colonic mucosa was measured by qRT-PCR analysis. GAPDH was used as an internal control to normalize the expression.

qRT-PCR, quantitative real-time polymerase chain reaction; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; M_Con., male control mice; M_ AOM/DSS, male mice treated with AOM/DSS; M_AOM/DSS/E2, AOM/DSS-treated male mice administered estradiol; F_Con., female control mice; F_AOM/DSS, female mice treated with AOM/DSS. *p<0.05 for comparison between two groups.