Table 1.
List of Oligonucleotides for qRT-PCR
| Gene | Sequence (5′→3′) |
|---|---|
| MUC2 | F: TTT CAA GCA CCC CTG TAA CC |
| R: AGG TCC TGG TGT TGA ACC TG | |
| MUC4 | F: CCC CCA TCT TTC TGT CTC AA |
| R: AGG ATG GAA TTG GTG TT TG | |
| ZO-1 | F: ACT ATG ACC ATC GCC TAC GG |
| R: GGG GAT GCT GAT TCT CAA AA | |
| OCLN | F: CGG TAC AGC AGC AAT GGT AA |
| R: CTC CCC ACC TGT CGT GTA GT | |
| CLDN4 | F: GGG GAT CAT CCT GAG TTG TG |
| R: CAC TGC ATC TGA CCT GTG CT | |
| KLF4 | F: CCA AAG AGG GGA AGA AGG TC |
| R: CTG TGT GAG TTC GCA GGT GT | |
| TNF-α | F: ACG GCA TGG ATC TCA AAG AC |
| R: GTG GGT GAG GAG CAC GTA GT | |
| IL-6 | F: CTG CAA GAG ACT TCC ATC CAG TT |
| R: GAA GTA GGG AAG GCC GTG G | |
| IL-1β | F: CAG GCA GGC AGT ATC ACT CA |
| R: TGT CCT CAT CCT GGA AGG TC | |
| ERα | F: TGG GCT TAT TGA CCA ACC TAG CA |
| R: AGA ATC TCC AGC CAG GCA CAC | |
| ERβ | F: TCT TTG CTC CAG ACC TCG TT |
| R: AAT CAT GGC CTT CAC ACA CA | |
| GAPDH | F: TTC ACC ACC ATG GAG AAG GC |
| R: GGC ATG GAC TGT GGT CAT GA |
qRT-PCR, quantitative real-time polymerase chain reaction; MUC2, mucin 2; MUC4, mucin 4; ZO-1, zonula occludens 1; OCLN, occludin; CLDN4, claudin 4; KLF4, Krüppel-like factor 4; TNF-α, tumor necrosis factor-α; IL, interleukin; ER, estrogen receptor; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.