Fig. 5.

Effects of TAK-875 on cell viability and ROS generation in a human dermal fibroblast (2D monolayer). Cells were exposed to TAK-875 (50, 100, 250 or 500 μM) or 0.1% SDS for 24 h and (A) cell viability was measured by MTT assay. (B) ROS generation was evaluated using DCF-DA enhanced fluorescence (100x magnification). H₂O₂ 100 μM for 10 min as a positive control for ROS generation. Data are mean ± SE of at least 3 replications. ^**p<0.01.