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. 2018 Nov 6;9(87):35726–35741. doi: 10.18632/oncotarget.26284

Figure 4. miR-198 and -206 inhibit both the migrative and invasive capabilities of the osteosarcoma cells in vitro.

Figure 4

(A) The migrative capabilities of the Osteosarcoma cells were assessed by migration assays in Boyden Chambers. The HOS LucF-GFP cells were transfected with the indicated pre-miRs (left panels) or anti-miRs (right panels) and 30 000 cells were seeded on the upper surface of the 8 µm-pored-Chambers in a 1% FBS-containing medium. The cells that had migrated to the lower surface of membranes after 24 h of incubation were counted. Histograms show the percentage of the lower membranes’ surface occupied by cells / the total lower membranes’ surface. Representative pictures were taken. Error bars show s. e. m. for n = 3 measurements from representative experiments. (B) The invasive capabilities of the cells were assessed as the same way as presented in (A), the upper surfaces of the Boyden Chambers were preliminary coated with 50 ng of Matrigel. (C) The viability of the cells was assessed through WST-1 assays in HOS LucF-GFP cells 72 h after the cells’ transfections with the pre-miRNAs -198, -206 or controls (Ct). *p < 0.05, **p < 0.01, ***p < 0.001. Error bars show s. e. m. for n = 3 measurements from representative experiments. One-way ANOVA followed by Dunnett’s multiple comparison test was used to assess the significance of the results in the experiments presented here.