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. 2018 Nov 9;9(88):35844–35855. doi: 10.18632/oncotarget.26312

Figure 1. Tankyrase is associated with the BRE-BRCC36-MERIT40 complex.

Figure 1

(A) List of tankyrase-binding proteins identified by liquid chromatography/mass spectrometry (LC-MS) analysis. FLAG-tagged tankyrase was overexpressed in HEK293T cells, and the cell lysates were immunoprecipitated with anti-FLAG antibody. The immunocomplexes were subjected to LC-MS analysis. (B) Top: tankyrase-binding motif (TBM) in TNKS1BP1 and MERIT40. Bottom: schematic diagram of MERIT40 mutants used in this study. Dark and light gray boxes indicate putative tankyrase-binding motifs. (C) Association of endogenous tankyrase and MERIT40 in A549 cells. A549 cells were immunoprecipitated (IP) with anti-MERIT40 antibody and subjected to western blot analysis with the indicated antibodies. Normal mouse immunoglobulin G (IgG) was used as negative control. (D) Association of tankyrase with Myc-tagged MERIT40 constructs. Myc-tagged MERIT40 was transiently expressed in HeLa cells and immunoprecipitated with anti-Myc antibody, followed by western blotting with anti-tankyrase, Myc, BRCC36 and BRE antibodies. Single and double asterisks indicate non-specific band and IgG heavy chain, respectively.