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. 2018 Nov 9;9(88):35844–35855. doi: 10.18632/oncotarget.26312

Figure 2. Tankyrase inhibitors sensitize A549 cells to X-ray irradiation.

Figure 2

(A) Tankyrase accumulation upon treatment with tankyrase inhibitors. A549 cells were treated with tankyrase inhibitors (XAV939, JW55, TNKS656 and G007-LK) or a PARP1/2 inhibitor (olaparib) at 3 μM for 16 h and then examined by western blot analysis. Actin served as a loading control. (B) Effects of tankyrase inhibitors on A549 cell sensitivity to X-ray irradiation. Cells were treated with the indicated tankyrase inhibitors or the PARP1/2 inhibitor olaparib at 3 μM for 16 h, followed by X-ray irradiation at 2, 4 or 6 Gy. After a 10-day incubation, the numbers of colonies were quantified. Data were normalized with the colony numbers at 0 Gy, in which cell viability was defined as 100%. Three independent experiments were performed and each experiment was performed in triplicate.