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. 2018 Nov 26;14(11):e8371. doi: 10.15252/msb.20188371

Figure 6. Comparison of mapping depth using adaptive evolution and the deep scanning mutagenesis library.

Figure 6

  1. Map of SNPs positions observed in each sequenced genome after 48‐h adaptation (n = 9) and 5‐day adaptation with one passage per day (n = 6). SNPs were mapped to the parent strain, sequenced after growth in minimal media in the absence of AEC.
  2. Categories of the SNPs found in Fig 6A, with the categories from genes that are directly linked to the lysine pathway highlighted in green.
  3. Circos plot of the SNPs found after 48‐hour adaptation relative to the lysine metabolism genes. The bar plots of each SNP represent the frequency across sequenced strains (n = 9). Lysine metabolism genes are highlighted as black bars (50× zoom). The inner green plot represents the average sequencing coverage for each position across all sequenced genomes.
  4. Map of enriched mutations found for the same selective pressure (1,000 μM AEC) using our deep scanning mutagenesis library. The bar plots represent log2 enrichment scores.