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. 2018 Oct 25;54(1):41–52. doi: 10.3892/ijo.2018.4607

Figure 6.

Figure 6

Cell migrational potency, epithelial-mesenchymal transition, and the expression of p-FAK, p-AKT and MMPs is suppressed by a FAK inhibitor in SphK1-overexpressing HT29 cells. (A) Survival rates of HT29 cells and western blot analysis. GAPDH was used as an internal control. (B) Reverse transcription-polymerase chain reaction analysis of target molecules. GAPDH was used as a reference and the SphK1(+)-HT29 was set to 1. (C) Western blot analysis. GAPDH was used as an internal control. (D) Microvilli and pseudopodia of SphK1(+)-HT29 and PF-SphK1(+)-HT29 cells under scanning electron microscopy (magnification, ×5.00k). (E) Cell migrational potency demonstrated by a wound healing assay (magnification, ×100) and analysis. Cell migrational potency demonstrated by a Transwell assay (magnification, ×200) and analysis. All data are presented as the mean ± standard deviation of three independent experiments. p, phosphorylated; FAK, focal adhesion kinase; AKT, protein kinase B; MMP, matrix metalloproteinase; SphK1, Sphingosine kinase 1; Con, control; PF, PF-562271.