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. 2018 Oct 24;54(1):29–40. doi: 10.3892/ijo.2018.4605

Figure 1.

Figure 1

Breast cancer cells and endothelial cells establish a bidirectional dialogue in co-culture models. (A) Endothelial MSS-31 cell proliferation was measured after a 3-day culture in 70% complete medium + 30% MMT neo cancer cell conditioned media (gray bar) or corresponding vehicle (white bar); n=3; *P<0.05. (B) MSS-31 cells were cultured in serum-free 3D matrix gels in absence or presence of MMT neo cell conditioned media. Images were acquired after a 10-day culture. (C) Fluorescent diO-labeled endothelial cells were cultured upon a 3D matrix gel containing or not cancer cells for 5 days. (D) MMT neo cancer cells were embedded within the 3D matrix gel, in absence or presence of endothelial MSS-31 cells on top of the gels. Images were acquired at the level of cancer cells in the gel. (E and F) DiI-labeled MMT cells (red fluorescence) form connected structures with diO-labeled MSS-31 cells (green fluorescence) in 3D co-cultures, observed at (E) 3 days or (F) 8 days after cell seeding. Labeling weakening over time does not allow a proper and unambiguous identification of cell type at heterotypic contact sites. Scale bars, 50 µm.