Table 1.
Isozyme | RFLP | RAPD | AFLP | SSR | SNP | |
---|---|---|---|---|---|---|
Abundance | Low | Medium | Very high | Very high | High | Very high |
Types of polymorphism | Amino acid change in polypeptide | Single base change, insertion, deletion, inversion | Single base change, insertion, deletion, inversion | Single base change, insertion, deletion, inversion | Repeat length variation | Single base change |
DNA quality | - | High | Medium | High | Medium | Medium |
DNA sequence information | - | Not required | Not required | Not required | Required | Required |
Level of polymorphism | Low | Medium | High | High | High | High |
Inheritance | Co-dominance | Co-dominance | Dominance | Dominance | Co-dominance | Co-dominance |
Reproducibility | Medium | High | Low | Medium | High | High |
Technical complexity | Medium | High | Low | Medium | Low | Medium |
Developmental cost | Medium | High | Low | Low | High in start | High |
Species Transferribility | High | Medium | High | High | Medium | Low |
Automation | Low | Low | Medium | Medium | High | High |
Note: Data on the RFLP, RAPD, AFLP and SSR were modified from Semagn et al. [48].