Figure 5.

Overexpression of miR-132 reverses EMT. (A) AsPC-1, PANC-1 and ASAN-PaCa cells were transfected with 50 nM miR-132 mimics, anti-miR-132 or negative miR control (NC) or were treated with 1 µM DEX in parallel or at 8 h after transfection in the presence or absence of miR-132 or anti-miR-132. At 48 h later, expression of E-cadherin and vimentin was examined via TaqMan miR real-time qPCR, as described in Fig. 2D. (B) AsPC-1 cells were transfected and treated with DEX as aforementioned, followed by protein isolation and western blot analysis using antibodies specific for E-cadherin and vimentin. β-actin was used as an internal control. The results for part A are shown as means. EMT, epithelial mesenchymal transition; miR, microRNA.