Fig. 4.

IBs to detect HDACs in control and Vorinostat-treated day 13 raft cultures. (A) IBs of lysates of HPV-18 and PHK raft cultures reveal steady-state levels of HDAC-1, HDAC-2, HDAC-3, and HDAC-4. The cultures were exposed to vehicle (0) or Vorinostat at three concentrations, as indicated, from days 6 to 13. Lysates from UT PHK raft cultures served as one of the controls. The signals were detected with ECL. (B) HDACs-5, HDAC -6, HDAC -7, and SirT-1 (in red) and actin (in green) from the same raft cultures were documented with the LI-COR CLx system. Brackets indicate blots from the same gel. Panels showing bands for H4K12Ac in Fig. 3B as well as bands of HDAC2 in A and HDAC5 in B (each marked with an asterisk) were detected in strips from the same gel.