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. 2018 Nov 1;115(47):E11138–E11147. doi: 10.1073/pnas.1801156115

Fig. 5.

Fig. 5.

IBs and IF to detect steady-state levels of HPV-18 E6 and E7 and target host proteins. (A and B) Two sets of independent day 13 HPV-18 cultures were exposed to the indicated Vorinostat concentrations from days 6 to 13. UT PHK or HPV-18−infected raft cultures exposed to vehicle (0) served as references. Actin was loading control. The ECL detection system was used to detect (A) p130, HPV-18 E6 and E7, and actin, (B) pRB, p53, and actin, (D) total ATM, phosphorylated ATM S1981, and Nbs1, (E) Mre-11 and actin, and (F) total (Upper) and phosphorylated (Lower) DNA-PKcs. (C) E6AP and actin were detected and documented with LI-COR CLx system. Images in A–C and E each represent blots from a separate gel. Protein bands identified in D and F are from the same gel as those in A and B, respectively. (G) PCNA (red) and cyclin B1 (green) were detected by IF in FFPE sections from one set of the infected cultures, exposed to DMSO (Left) or 5 μM Vorinostat (Right). A and F are from the same gel and had same actin loading control, indicated by a single asterisk (*) in A. ** and *** indicate actin loading controls of B and C IBs.