Fig. 8.

IBs and IF to detect steady-state levels of the proapoptotic proteins. (A) Apaf1, PUMA, Bax, and Noxa. IBs marked with ** and *** are from the same gels as shown in Fig. 5 B and C, which included the actin loading control. (B) IBs to detect EZH2 (Upper) and Bim (Lower) in day 13 lysates from UT PHK and HPV-18 cultures as well as HPV-18 raft cultures exposed to Vorinostat from days 6 to 13 at the indicated concentrations. EL, L, and S indicate extralong, long, and small forms of Bim, respectively. The blots are from the same gels as presented in Figs. 5E and 6C, where the actin loading controls are shown. (C) Indirect IF detection of Bim protein (green) in tissue sections of HPV-18 raft cultures.