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. 2018 Nov 5;115(47):E11178–E11187. doi: 10.1073/pnas.1811491115

Fig. 3.

Fig. 3.

The NBE sequence in the 5′ UTR of the NCED3 promoter was essential in inducing expression of the reporter gene during dehydration stress in planta. (A) Schematic diagrams of various NCED3 promoters that drive a GUS reporter gene. (B) Expression levels of NCED3 and GUS reporter in the transgenic plants under dehydration-stress conditions (Dry). B, Upper indicates the relative expression levels of GUS during dehydration stress compared with that of the 3 kbp:GUS plants under nonstress conditions. B, Lower indicates the expression levels of the endogenous NCED3 genes as a control. The error bars indicate the SD from triplicate technical repeats. (C) Fold increases of relative expression of the reporter gene during dehydration stress. The fold increases of the reporter expression levels at 4 h of dehydration stress relative to those under control conditions (0 h) in each plant line are shown. The error bars indicate the SD from triplicate technical repeats. The letters above the bars indicate significant differences between the plant lines at each time point (P < 0.05, according to Tukey’s multiple range test).