Fig. 5.

S55746 synergizes with panobinostat in vitro and in vivo in DLBCL. (A) Western blot showing increase in NOXA protein levels and decrease in MCL1, after treatment with the combination of panobinostat and S55746 for 24 h. (B) Heat maps showing the effect of the combination of S55746 and panobinostat on cell viability. Percentage of cell viability is depicted in a colorimetric scale from red (high) to green (low) normalized to DMSO (control). Values are the mean ± SD of three separate determinations. Cells were incubated with increasing concentrations of S55746 and panobinostat for 24 h and cell viability was determined by Celltiter-Glo assay. (C) NSG mice (n = 8 per treatment group) were injected with DLBCL PDX and with either vehicle, panobinostat(5 mg/kg five times weekly), UMI-77 (60 mg/kg every other day), S55746 (75 mg/kg five times weekly), or the two drugs together for 3 wk and observed until death after the end of the treatment. Differences among groups were calculated with the ANOVA with Dunnett’s test. *P = 0.04, **P = 0.003, ***P = 0.0007, ****P < 0.0001.