Figure 1. Inhibition of endocytosis in glia produces resistance to mechanical sleep deprivation.

(A) Resistance to mechanical sleep deprivation, manifest as elevated sleep during stimulation, for Repo-GAL4 > UAS Shi.ts1;UAS-Shi.ts1 female flies (n = 14–16, per genotype) and male (n = 16, per genotype) at both 30°C and 18°C. Dashed box indicates the period of mechanical deprivation, ZT12-24. Red shading indicates 30°C, otherwise the temperature is 18°C. (B) Sleep during deprivation at permissive temperature (18°C) was also present using an additional Shi¹ line, in Repo-GAL4 > UAS-20xShi.ts1 females (n = 16, per genotype) and males (n = 16, per genotype). One-way ANOVA with Holm-Sidak post-hoc test, *p<0.05, **p<0.01, ***p<0.001. Error bars represent standard error of the mean (SEM).

Figure 1—figure supplement 1. Glial Shi¹ expression does not affect responsiveness to mechanical stimulus during wake.

(A) Mean activity (beam breaks) of Repo-GAL4 > UAS-20xShi.ts1 and control female flies at 18°C between ZT11 and 12, during mechanical stimulation (dashed box) as used for sleep deprivation (n = 16, each genotype). One-way ANOVA with Holm-Sidak post-hoc test, *p<0.05, **p<0.01, ***p<0.001. Error bars represent standard error of the mean (SEM).