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. 2018 Nov 26;7:e43326. doi: 10.7554/eLife.43326

Figure 2. Inhibition of endocytosis in glia increases baseline sleep.

(A) Daily baseline sleep amount (3 day mean) and sleep/activity characteristics including mean daytime sleep bout number, bout length, and activity index for Repo-GAL4 > UAS Shi.ts1;UAS-Shi.ts1 female flies at 18°C (n = 19–26), and (B) Repo-GAL4 > UAS-20xShi.ts1 females (n = 23–31). (C) Daytime sleep for female flies at 18°C expressing either a constitutively dominant negative Shi (UAS-Shi.K44A) (n = 15–16) or a wild-type Shi (UAS-Shi.WT) (n = 15–32) by Repo-GAL4. One-way ANOVA with Holm-Sidak post-hoc test, *p<0.05, **p<0.01, ***p<0.001. Error bars represent standard error of the mean (SEM).

Figure 2.

Figure 2—figure supplement 1. Inhibition of endocytosis in glia increases baseline sleep in males.

Figure 2—figure supplement 1.

(A) Daily baseline sleep amount (3 day mean) and sleep/activity characteristics including mean daytime sleep bout number, bout length, and activity index for Repo-GAL4 > UAS Shi.ts1;UAS-Shi.ts1 male flies at 18°C (n = 24–26), and (B) Repo-GAL4 > UAS-20xShi.ts1 males (n = 28–31).
Figure 2—figure supplement 2. Shi1 expression at 30°C in glia increases baseline sleep similar to expression at 18°C.

Figure 2—figure supplement 2.

(A) Daily baseline sleep amount for Repo-GAL4 > UAS-20xShi.ts1 female flies at 30°C (2 day mean), n = 23–31. One-way ANOVA with Holm-Sidak post-hoc test, *p<0.05, **p<0.01, ***p<0.001. Error bars represent standard error of the mean (SEM). (B) Total sleep of Repo-GAL4 > UAS-20xShi.ts1 females flies at 18°C (mean of 3 days) and 30°C (mean of 2 days) (n = 30). Unpaired t-test. (C) Daily baseline sleep amount for Repo-GAL4 > UAS-20xShi.ts1 male flies at 30°C (2 day mean), n = 28–31. One-way ANOVA with Holm-Sidak post-hoc test, *p<0.05, **p<0.01, ***p<0.001. Error bars represent standard error of the mean (SEM). (D) Total sleep of Repo-GAL4 > UAS-20xShi.ts1 males flies at 18°C (mean of 3 days) and 30°C (mean of 2 days) (n = 30). Unpaired t-test.
Figure 2—figure supplement 3. Inhibition of endocytosis in glia increases total baseline sleep.

Figure 2—figure supplement 3.

(A) Total baseline sleep amount (3-day mean) for Repo-GAL4 > UAS Shi.ts1;UAS-Shi.ts1 female flies at 18°C (n = 19–26), Repo-GAL4 > UAS-20xShi.ts1 females (n = 23–31), Repo-GAL4 > UAS Shi.ts1;UAS-Shi.ts1 male flies at 18°C (n = 24–26), and Repo-GAL4 > UAS-20xShi.ts1 males at 18°C (n = 28–31). (B) Total sleep time for female flies at 18°C expressing either a constitutively dominant negative Shi (UAS-Shi.K44A) (n = 15–16) or a wild-type Shi (UAS-Shi.WT)(n = 15–32) by Repo-GAL4. One-way ANOVA with Holm-Sidak post-hoc test, *p<0.05, **p<0.01, ***p<0.001. Error bars represent standard error of the mean (SEM).