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. 2018 Aug 31;69(22):5587–5597. doi: 10.1093/jxb/ery319

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© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 1. — A lipid transfer protein (LTP) is the major protein in Brassica rapa nectar. (A) Whole B. rapa flower (left) beside one from its close relative, Arabidopsis. (B) Example of a nectar droplet collected from B. rapa flowers for protein identification. LN, lateral nectary; Ov, ovary; Pe, petal. A short stamen was removed from the flower to visualize the nectar droplet. (C) Protein profile of raw B. rapa nectar after separation by 12% SDS-PAGE and silver staining. The major protein band (arrowhead) was excised from the gel and processed for protein identification. (D) Peptides identified from the major protein band [arrowhead in (C)] by LC-MS/MS. (E) BLAST searches identified the major protein band as Bra028980, a putative lipid-transfer protein. Peptides identified by MS/MS are shaded in gray, cysteines are highlighted, and a putative signal peptide required for secretion from the cell is underlined.