Table 2.
Identification of proteins with altered abundance in dapat with respect to the WT at the end of the day
Spot | Locus | Name | Fold | P-value |
---|---|---|---|---|
1 | At2g45790 | Phosphomanomutase | NDa | 0.0021 |
2 | At3g48870 | HSP93-III | NDa | 0.0244 |
3 | AtCg00490 | Rubisco large chain | 7.1519 | 0.0228 |
4 | At3g55800 | Sedoheptulose-1,7-bisphosphatase | 5.8696 | 0.0083 |
5 | AtMg01190 | ATP synthase subunit 1 | 4.0432 | 0.069 |
6 | At5g13850 | NACA3 | 1.7853 | 0.0459 |
7 | At2g36880 | S-Adenosylmethionine synthase 3 | 1.7631 | 0.0107 |
8 | At1g57720 | Elongation factor EF1B | 1.7075 | 0.0397 |
9 | At4g02520 | Glutathione S-transferase 2 | 1.6004 | 0.0069 |
10 | At1g42970 | Glyceraldehyde-3-phosphate dehydrogenase subunit b | 1.5973 | 0.0083 |
11 | At3g01500 | Beta carbonic anhydrase 1 | 1.5916 | 0.0193 |
12 | At3g59970 | Methylenetetrahydrofolate reductase 1 | 1.5300 | 0.0500 |
13 | At4g16143 | Importin α isoform 2 | 1.4309 | 0.0242 |
14 | At1g09780 | Phosphoglycerate mutase | 1.3960 | 0.064 |
15 | At5g25980 | Glucoside glucohydrolase 2 | 1.3851 | 0.0445 |
16 | At2g45140 | Lipoxygenase 2 | 1.3793 | 0.060 |
17 | At2g27720 | 60S acidic ribosomal protein | 1.3676 | 0.0329 |
18 | At4g04640 | ATPase γ chloroplastic | 1.3638 | 0.0288 |
19 | At5g42020 | BiP2 | 1.2800 | 0.0034 |
20 | At1g23310 | Ala:2-OG aminotransferase 1 | 1.2626 | 0.0125 |
21 | At2g30860 | Glutathione S-transferase 9 | 1.2419 | 0.0130 |
22 | At3g11630 | 2-Cys peroxiredoxin | 1.2416 | 0.0357 |
23 | At2g47730 | Glutathione S-transferase 8 | 1.1736 | 0.0016 |
24 | At2g45140 | Lipoxygenase 2 | 1.1517 | 0.0142 |
25 | At1g20020 | Ferredoxin–NADP reductase 2 | 1.1364 | 0.0031 |
26 | At5g24490 | 30S ribosomal protein | 0.8957 | 0.0130 |
27 | At3g55440 | Triose phosphate isomerase | 0.8886 | 0.0690 |
28 | At3g01500 | Beta carbonic anhydrase 1 | 0.8596 | 0.0344 |
29 | At4g25100 | Fe-superoxide dismutase | 0.8483 | 0.0112 |
30 | At1g32060 | Phosphoribulokinase | 0.8449 | 0.0075 |
31 | At3g01500 | Beta carbonic anhydrase | 0.8194 | 0.0032 |
32 | At3g11630 | 2-Cys peroxiredoxin | 0.7797 | 0.0574 |
33 | At4g24280 | HSP70 | 0.7579 | 0.0292 |
34 | At3g09440 | HSP70 protein 3 | 0.7367 | 0.0163 |
35 | At1g01090 | Pyruvate dehydrogenase E1α | 0.7242 | 0.0592 |
36 | At3g01500 | Beta carbonic anhydrase 1 | 0.7216 | 0.0293 |
37 | At3g50820 | Oxygen envolving complex | 0.7202 | 0.0355 |
38 | At3g15360 | Thioredoxin M-type 4 | 0.7151 | 0.0401 |
39 | At5g39570 | Uncharacterized protein | 0.6994 | 0.0310 |
40 | At5g25980 | Beta glucosidase 37 | 0.6862 | 0.0012 |
41 | At4g01850 | S-Adenosylmethionine synthase 2 | 0.6586 | 0.0493 |
42 | At2g39730 | Rubisco activase | 0.6551 | 0.0580 |
43 | At1g68010 | Hydroxypyruvate reductase 1 | 0.6392 | 0.0456 |
44 | At2g38230 | Pyridoxine biosynthesis 1 | 0.6208 | 0.0107 |
45 | At5g06290 | 2-Cys peroxiredoxin | 0.5735 | 0.0547 |
46 | At3g63540 | Thylakoid lumenal 19 kDa | 0.5616 | 0.0337 |
47 | At5g53490 | Thylakoid lumenal 17.4 kDa | 0.5429 | 0.0195 |
48 | At1g54270 | eIF4A | 0.5140 | 0.0441 |
49 | At5g52310 | Responsive to dessication 29A | 0.5124 | 0.0197 |
50 | At5g39570 | Uncharacterized protein | 0.3539 | 0.0469 |
51 | At5g17920 | Methionine synthesis 1 | 0.3509 | 0.0051 |
52 | At3g52960 | Thioredoxin superfamily protein | 0.0886 | 0.0030 |
53 | At1g31180 | Isopropylmalate dehydrogenase 3 | NDb | <0.001 |
54 | At1g78330 | Glutathione S-transferase 19 | NDb | <0.001 |
55 | At3g50820 | Oxygen envolving complex | NDb | <0.001 |
56 | At1g53850 | 20S Proteasome α subinit E1 | NDb | 0.0069 |
57 | At2g34430 | LCB-II | NDb | 0.0072 |
58 | At3g26650 | Glyceraldehyde-3-phosphate dehydrogenase subunit a | NDb | 0.0252 |
59 | At1g67090 | Rubisco small chain | NDb | 0.0254 |
60 | At2g34430 | LCB-II | NDb | 0.0396 |
Proteins were separated by IEF/SDS-PAGE and spots were analysed by MALDI TOF/TOF (n=3). P<0.05.
a ND: not detected in wild-type (Col-0).
b ND: not detected in dapat mutant.