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. 2018 Nov 15;19(1):foy120. doi: 10.1093/femsyr/foy120

Figure 6.

Figure 6.

The short 5΄ ETS rRNA transcripts are not transcribed by RNA polymerase II. (A) Northern blot of total RNA from cells expressing the rDNA from a cryptic RNA pol II promoter (PSW/ NOY878) or from plasmid-encoded pPol I rDNA (NOY908) and pPol II rDNA (NOY891). The blot was probed with radiolabeled +107 oligonucleotide. (B) Northern blot of total RNA from yeast grown in galactose for 0, 3 and 6 hours to deplete UTP7 and UTP15 and hybridized to detect the short transcripts. (C) Northern blot of total RNA from YPH499 cells grown at 30°C in YPD then shifted to 37°C or media with no dextrose (YP) or grown to stationary phase (OD600 5.5). (D) ChIPs with 5΄ETS primers of Utp3 and Utp15-HA in NOY504, carrying a temperature sensitive RNA pol I were shifted to 37°C for 7 hours to repress rDNA transcription. Input DNA was diluted 2.5-fold.