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Pol5. Primers to the 5΄ETS amplified chromatin isolated from each tagged strain, normalized to ACT1 and standard deviation noted. (C) Northern blot of short RNAs when Pol5, Utp7 and Utp10 were depleted for 24 hours. (D) Northern blot of RNA extracted from anti-HA immunoprecipitations. HA-tagged proteins were immunoprecipitated and RNA was separated on an 8% polyacrylamide gel. Oligonucleotide +107 was radiolabeled and blotted to detect the short 5΄ ETS pre-rRNA transcripts.
