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. 2018 Nov 15;19(1):foy120. doi: 10.1093/femsyr/foy120

Table 2.

Strain list.

Strain Genotype Reference
YPH499 MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1 (Sikorski and Hieter 1989)
YPH500 MATα ura3-52 lys2-801_amber ade2-101_ochre trp1-Δ63 his3-Δ200 leu2-Δ1 (Sikorski and Hieter 1989)
YPH499 HA C-terminal 3xHA tag (Dragon et al.2002)
YPH499 TAP C-terminal TAP tag (TRP1) (Gallagher et al.2004)
YKW100 MATa, ura3-52, his3-Δ, leu2 lys2-801 amber, trp1-Δ6,3 u3aΔ UAS GALpro:U3A::URA3 u3b::LEU2 (Wehner et al.2002)
YJV100 MATα, ade3-1, his3-11, leu2-3,112, trp1-1, ura3-1 can100, rpa135::LEU2, GAL7-35rDNA::TRP1d (Venema et al.1995)
K3207 MATa his4-260::URA3, his4−Δ ade2-1, ade5 ura3-52, HOT1::leu2::ADE5,7::leu2-3,112, trp1HIII, lys2-BX::CAN1::LYS2, can1 (Lin and Keil 1991)
YPH499 GAL:HA GAL1 driven N-terminal 3xHA tag (KANR) (Dragon et al.2002)
NOY504 MATα, ade2-101, ura3-1, trp1-1, leu2-3,112,his3-11, can1-100, rrn4::LEU2, (pol I temperature sensitive) (Nogi et al.1993)
NOY878 MATa, ade2-101, ura3-1, trp1-1, leu2-3,112, can1-100, rrn9::HIS3, PSW (35S rDNA promoter switch from pol I to pol II) (GAL7-35S rDNA, 5S rDNA, URA3, 2 μm (pNOY103)) (Oakes et al.1999)
NOY891 MATa ade2-101, ura3-1, trp1-1, leu2-3,112, can1-100, rdnΔΔ::HIS3 carrying pNOY353 (GAL7-35S rDNA, 5S rDNA, TRP1, 2 μm (pNOY353)) (Oakes et al.1999)
NOY908 MATa ade2-101, ura3-1, trp1-1, leu2-3,112, can1-100, rdnΔΔ::HIS3 carrying pNOY373 (pol I rDNA LEU2, 2 μm (pNOY373) (Wai et al.2001)
pPol II rDNA NOY891 with pRS315 This study
pPol I rDNA NOY891 with pNOY373 This study
pdeletions+ pPol II rDNA NOY891 with pNOY373 with different length deletions of the 5΄ETS (long, medium or short 5’ETS transcripts) This study