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. 2018 Aug 22;69(22):5573–5586. doi: 10.1093/jxb/ery310

Fig. 1.

Fig. 1.

Protein alignment of StLRPK1 with Arabidopsis SRF family protein sequences. (A) Alignment of the amino acid sequences of SRF full-length proteins and the StSERK3A kinase domain. Full conservation across the kinase domain alignment is marked by black columns and partial conservation by gray columns. The predicted signal peptide sequences and the kinase subdomain are underlined with thick pink and red lines, respectively. The SUB domains, the transmembrane domains, and the proline-rich regions are marked with black, yellow and blue rectangles, respectively. The six LRRs are marked with green lines above each LRR region. Two red rectangles indicate the regions containing two typical HxD and DFG motifs in active kinases, corresponding to the VI and VIIa subdomains of the StSERK3A/BAK1 kinase domain. (B) Schematic representation of the StLRPK1 structure. LRR, leucine-rich repeat; PRR, proline-rich region; SP, signal peptide; SUB, SUB domain; TM, transmembrane domain. (C) Phylogenetic tree of the SRF family. A maximum likelihood tree obtained using the amino acid sequences of the potato StLRPK1 and Arabidopsis SRF members as input. The branch support values are indicated. The protein GenBank accession numbers for alignments are as follows: StLRPK1 (EU049848), Arabidopsis SRF1 (AY518286), SRF2 (AY518287), SRF3 (AY518288), SRF4 (AY518289), SRF5 (AY518290), SRF6 (AY518291), SRF7 (AY518292), SRF8 (AY518293), SRF9 (AF399923, SUB), and StSERK3A/BAK1 (AGT21432.1).