Fig. 2.
StLRPK1 transcript abundance in response to flg22 and CF, and the localization of StLRPK1-GFP. (A) Expression of StLRPK1 in response to flg22 and P. infestans culture filtrate (CF) treatment. Leaves on intact potato plants were pressure infiltrated with P. infestans CF or medium as a control, or flg22 or double-distilled H2O (ddH2O) as a control. Treated leaves were collected at 0, 0.5, 1, 3, and 6 h. qRT-PCR was performed to test the gene expression level. Data represent three biological replicates. (B) Representative confocal images of StLRPK1-GFP localization. Transient expression of StLRPK1-GFP in transgenic N. benthamiana expressing the plasma membrane (PM) marker mOrange-LTi6 or co-expression of StLRPK1-GFP with the cytoplasmic marker mRFP. From left to right: the green channel (StLRPK1-GFP), the merged channel, and the orange channel (mOrange-Lti6) or red channel (mRFP). The plots of fluorescence profiles (to the right of the confocal images), represented by the white arrows in the two merged images, indicate clear co-localization of StLRPK1-GFP with the PM marker but no co-localization with free mRFP. Scale bars=10 μm.