Fig. 7.
Silencing of NbBAK1 compromises the ability of StLRPK1 to inhibit P. infestans colonization in N. benthamiana. (A) Representative image of N. benthamiana plants after VIGS of NbBAK1. OE1 and OE9 are two transgenic homozygous lines ectopically overexpressing StLRPK1. (B) Silencing efficiency is shown by the mean fold change measured by qRT-PCR of three biological replicates, using N. benthamiana plants 3 weeks post-inoculation with TRV:GFP and TRV:BAK1 vectors. (C) Mean P. infestans lesion diameter measured at 6 dpi in a sample of approximately 60 leaves (approximately120 lesions) for each construct in each VIGS background from four biological replicates. P. infestans isolate 88069 sporangia were used to inoculate leaves. Values labeled with different letters are statistically different by one-way ANOVA using pairwise multiple comparison procedures with the Holm–Sidak method (P<0.01). Error bars represent ±SD.
